The windscreen of Aston Villa's team bus was hit by a brick as the side were travelling back to the Midlands following their victory at Burnley.
The incident took place at junction 10 of the M65 after Sunday's Premier League match, which Villa won 3-1.
Police said the brick was thrown from a footbridge as the bus travelled along the motorway at around 17:15 BST.
In a statement Burnley said the club are "relieved to hear nobody was hurt in the incident".
"We strongly condemn this behaviour and will support Lancashire Police in their efforts to find whoever was responsible," the statement added.
Supt Melita Worswick, of Lancashire Police, said: "This incident occurred when a great deal of traffic was leaving the area following the football match between Burnley and Aston Villa.
"It is nothing but good fortune that the brick didn't cause more damage, or result in somebody being seriously injured or even killed."
The incident happened around two miles away from Burnley's Turf Moor ground.
The match was Burnley's second home game of the season. After their opening match against Premier League champions Manchester City, Burnley issued a statement condemning the "unacceptable behaviour" of some supporters for incidents including items being thrown and fans getting on to the pitch.
City defender Rico Lewis was struck by a lighter thrown from the crowd while a fan was restrained when they tried to get on to the playing area.