It remains the most expensive prize in world football, with the riches of Premier League television money afforded to top-flight clubs.
Deloitte says that if the Bees were to survive their first season the additional revenue would rise to £265m over five years, with Championship winners Leeds benefiting to the same tune as Brentford.
Fulham would receive around £135m over three years if they beat their fellow west Londoners, a reduced amount because they are currently in receipt of parachute payments after their relegation to the Championship in 2019.
Dan Jones, partner and head of the sports business group at Deloitte, said: "This season's Championship has been temporarily interrupted by the Covid-19 pandemic, however as the season comes to a close, all eyes will be on Wembley in what still remains world football's biggest financial, winner-takes-all, prize match.
"Despite the disruption to the season, the league has remained extremely competitive at the top-end of the table, and yet again the play-off semi-finals have provided supporters and neutral football fans with excellent entertainment."
Deloitte said the additional £160m is made up of an estimated £85m in central broadcasting distributions, and parachute payments over the 2021-22 and 2022-23 seasons totalling £75m if they are relegated next summer.
The director of Deloitte's sports business group Tim Bridge said: "The financial prize for Brentford or Fulham, as well as Leeds United and West Bromwich Albion, is likely to be in excess of £265m if they survive their first season in the Premier League.
"Furthermore, given the recent impact of Covid-19, it is likely that the financial impact of promotion will be better received than ever before.
"The increased revenue provides these clubs with the platform from which they can develop on and off the pitch.
"Promotion to the Premier League provides clubs with the financial resources to make strategic investments.
"While this typically includes increased expenditure in on-pitch playing talent, it still remains important that promoted clubs are committed to their longer-term financial responsibility."
Additional reporting by PA